Search results for "Biochemistry and Metabolism"

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Functional characterization of the plastidial 3-phosphoglycerate dehydrogenase family in Arabidopsis.

2013

This work contributes to unraveling the role of the phosphorylated pathway of serine (Ser) biosynthesis in Arabidopsis (Arabidopsis thaliana) by functionally characterizing genes coding for the first enzyme of this pathway, 3-phosphoglycerate dehydrogenase (PGDH). We identified two Arabidopsis plastid-localized PGDH genes (3-PGDH and EMBRYO SAC DEVELOPMENT ARREST9 [EDA9]) with a high percentage of amino acid identity with a previously identified PGDH. All three genes displayed a different expression pattern indicating that they are not functionally redundant. pgdh and 3-pgdh mutants presented no drastic visual phenotypes, but eda9 displayed delayed embryo development, leading to aborted emb…

PhysiologyMutantMolecular Sequence DataArabidopsisPlant SciencePlant RootsGene Expression Regulation EnzymologicSerineBiochemistry and MetabolismGene Expression Regulation PlantComplementary DNAArabidopsisGeneticsSerineArabidopsis thalianaMetabolomicsAmino Acid SequencePlastidsPhosphorylationGenePhosphoglycerate DehydrogenasePhylogenyTapetumMicroscopy ConfocalbiologySequence Homology Amino AcidArabidopsis ProteinsReverse Transcriptase Polymerase Chain ReactionGenetic Complementation Testfood and beveragesPlant Components Aerialbiology.organism_classificationPlants Genetically ModifiedPhenotypeIsoenzymesBiochemistryMultigene FamilyMutationSeedsPollenPlant physiology
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